The Combination of bFGF and Hydrocortisone is a Better Alternative Compared to 5-Azacytidine for Cardiomyogenic Differentiation of Bone Marrow and Adipose Stem Cells

Stem cells can be differentiated into cardiomyocytes by induction with 5-azacytidine (5-aza) but its carcinogenicity is of concern for future translational application. Alternatively, growth factors and hormones such as basic fibroblast factor (bFGF) hydrocortisone have been reported to act a therapeutic inducer differentiation. In this study, we aim investigate the ability bFGF in combination stimulate differentiation mesenchymal stem (MSC) lineage. Sheep adipose tissue cell (ATSC) bone marrow (BMSC) were isolated, cultured induced three groups factors; 5-aza alone, all cardiomyogenic Morphological, protein functional both ATSC BMSC observed analysed confirm cardiomyocyte Viability each treated group was significantly higher (P < 0.05) on after 10 nM 50 μM hydrocortisone. Cardiomyocyte proteins; α-Sarcomeric actin (αSA) Phospolamban (Plb) detected exposed not control negative group. Both without showed only minute number possesses αSA Plb. Calcium ion (Ca2+) spark primary heart cells. Similarly, Ca2+ also BMSC, proving some functionality conclusion, are safer compared currently used viability induction, therefore more available use cardiac engineering.